Application Project – BioPhenics

Identification

Name: *

First

Last: *

Last

Email: *

Phone:

Address: *

Address Line 2:

City: *

Postal Code: *

Country: *

General Information

People involved in the project (only name the person that will be directly in contact with the BFX team members): *

Name/Lab status/role

Project Leaders/Unit: *

Scientific Part

Main Objective: *

Addressed questions: *

What particular BFX resources would you need: *

(e.g. screening your library, your cell line, your particular assay, screening our chemical library, our siRNA library, assembling and screening a new library, …)

Project Summary (the question, present situation, the screen and afterward): *

Relation to Cancer (if any): *

Guidance for HTS Application

Assay applications will be evaluated in terms of the following characteristics:

Readiness for or adaptability to HTS: *

Are the assays ready to be used in micro-titre plates, such as 96-well ? Do you need a particular cell line ? Are steps such as centrifugation, filtration and extraction included in your protocol ? If yes, can they be substituted to comply with robotics handling ? Does it involved cell treatment before fixation (e.g hormones, heat shock, etc…). If yes, please outline the time frame.

Assay performance and robustness: *

Provide useful information on how well the assay performs in terms of reproducibility and robustness (e.g. between day-to-day experiments)

Brief description of the phenotype(s) followed: (tentative description) *

Note: Phenotypic assays measure a signal which corresponds to a complex response such as cell survival, proliferation, localization of a protein, nuclear translocation etc.

Technical part

Experimental system.

Cell line: (name, provider, number of passages tolerated) *

Inducers (ie ± TNF, IL-1, drugs): *

Describe specific antibodies used and /or fluorescently labelled dyes (name and source) *

Brief description of the labeling protocol: *

Choice of BFX perturbators library (which siRNA or chemical library) Vbis. Alternative perturbators: *

Planned Number of genes or components to be tested: *

Possible Controls (positive and negative phenotypic controls): *

Note: The selection of controls requires careful consideration and optimization as early as in assay development, as this substantially influences the quality and relevance of data and resulting knowledge.

Results of the preliminary experiments (upload a .zip or a pdf): *

Maximum file size: 67.11MB

Please provide images with detailed experimental conditions as well as a summary of results previously obtained (ie time-course, dose-titration, etc..)