The Institut Curie Screening Facility (BioPhenics) provides biochemical and cell-based screening support for investigators throughout the research community.
BioPhenics uses image-based high-content screening assays to evaluate multiple biochemical and morphological parameters in cells. Its technology allows tracking phenotypes at the cellular and subcellular levels under various perturbing conditions (chemical and siRNA collections).
Our mission is to support both basic and translational biomedical research by helping research teams in designing, optimizing, and running biochemical and cellular screening assays. BioPhenics is designed to function both as an intellectual base and a core research facility for researchers in their goals to discover gene functions and novel treatments for diseases.
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Most screening projects rely in two main categories:
- Primary research focusing on understanding basic mechanisms in cell biology: understanding the basic questions behind the biology of epithelial cells cultures in vitro like cell proliferation, trafficking of receptors, organelle morphology, reporter gene activation, and protein translocation events etc. Underlying mechanisms are examined using either commercially available cells cultured in vitro or cellular models developed by research groups. Although applicable to a wide range of targets, we have used this approach to measure the activity and selectivity of siRNA and chemical compounds in a large panel of phenotypic screening projects.
- Cancer biology and disease: Contribute to the understanding of cellular mechanisms involved in tumor cell survival and thus, contribute to the optimization of selective inhibitors for targeted therapy. Facilitate the repositioning of know compounds with biological activities in cancer cell-models. All our screening activity is based on visualization of the effect of small molecules on different disease-oriented cell models.
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